581 Hair histology and glycosaminoglycans distribution probed by infrared spectral imaging: Focus on heparan sulfate proteoglycan and glypican-1 during hair growth cycle

C Colin-Pierre,V Untereiner,G Sockalingum,N Berthelemy,L Danoux,V Bardey,S Mine,C Jeanmaire,L Ramont,S Brezillon

doi:10.1016/j.jid.2021.02.609

Abstract

Heparan sulfate proteoglycans (HSPGs) distribution in hair follicle (HF) is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this study, a novel approach is proposed to assess hair histology and HSPG distribution changes in the HF at different phases of the hair growth cycle using infrared spectral imaging (IRSI). Particularly, we were interested in the expression of glypican-1 in different HF compartments and their potential roles during hair shaft growth, as their roles are still poorly understood. The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. Findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs, as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia.

Highlights

In hair follicle organ culture model, selected peptide showed a hair shaft elongation activity. These results suggest that Wnt/b-catenin/DKK-1 signaling is important for hair cycle modulation and DKK-1 inhibiting peptide can be used for hair growth through b-catenin signaling stimulation
Dermal EZH2 orchestrates dermal differentiation and epidermal proliferation during murine skin development V Thulabandu1, T Nehila1, J Ferguson1 and R Atit1,2,3 1 Biology, Case Western Reserve University, Cleveland, Ohio, United States, 2 Genetics, Case Western Reserve University, Cleveland, Ohio, United States and 3 Dermatology, Case Western Reserve University, Cleveland, Ohio, United States Skin development and patterning is dependent on factors that regulate the stepwise differentiation of dermal fibroblasts concomitant with dermal-epidermal reciprocal signaling, two processes that are poorly understood
Our study reveals that dermal EZH2 acts as a rheostat to control the levels of Wnt/b-catenin and retinoic acid (RA) signaling to impact fibroblast differentiation cell autonomously and epidermal keratinocyte development non-cell autonomously, respectively

Summary

Introduction

Dermal EZH2 orchestrates dermal differentiation and epidermal proliferation during murine skin development V Thulabandu1, T Nehila1, J Ferguson1 and R Atit1,2,3 1 Biology, Case Western Reserve University, Cleveland, Ohio, United States, 2 Genetics, Case Western Reserve University, Cleveland, Ohio, United States and 3 Dermatology, Case Western Reserve University, Cleveland, Ohio, United States Skin development and patterning is dependent on factors that regulate the stepwise differentiation of dermal fibroblasts concomitant with dermal-epidermal reciprocal signaling, two processes that are poorly understood. Based on hair growth cycle, increasing the hair follicles in the anagen stage and decreasing the follicles in catagen and telogen stage is commonly adopted strategy for treating hair loss or alopecia.

Results

Conclusion