58] Labeling of antilactose antibody

Abstract

Publisher Summary Affinity labeling studies with anticarbohydrate antibodies have been very limited. In earlier studies, diazoniumphenyl glycosides were employed as affinity labeling reagents for rabbit and equine anti-p-azophenyl-β-lactoside and p-azophenyl- β-galactoside antibodies. Although these antibodies were heterogeneous, it was possible to identify the labeled residues in the heavy or light chains since the modified residues had characteristic absorption spectra. With the discovery of bacterial cell walls of Streptococcus groups A and C induced antipolysaccharide antibodies of restricted heterogeneityp such antibodies have been used for affinity labeling studies. This chapter describes the production of antilactose antibodies, synthesis of affinity labeling reagents containing lactose, the labeling conditions, and the distribution of label in the polypeptide chains of the antibodies. It explains the antilactose antibodies, synthesis of affinity labeling reagents, affinity labeling conditions, and distribution of label in the polypeptide Chains of the antibodies.