574 Three-dimensional culture of dermal papilla cells improves their in vitro angiogenic activity

Abstract

Tissue-engineered skin constructs correct vascularization remains a major challenge. We have shown that the presence of dermal papilla cells (DPCs) in these constructs favors the vascularization process, resulting in a better wound healing and graft take. To culture DPC as spheres increases the expression of angiogenic genes such as VEGF, angiogenin and FGF. Since angiogenesis in scaffolds is essential for grafts to survive and integrate with existing host tissue, our aim was to compare the ability of monolayer and sphere cultures of DPC to favor endothelial cell migration and neovessel development by using two angiogenic study models.