Abstract
Dextransucrase is a glucosyltransferase (GTF) produced by a variety of oral streptococci and other microorganisms. Several organisms produce multiple types of glucosyltransferases that are distinguished from one another by the structural characteristics of the polymers they form. The terms GTF(S) and GTF(I) have been used to designate the enzymes responsible for the formation of soluble and insoluble products. The reaction catalyzed by GTFs may play an important role in the formation of dental caries because both products are necessary participants in the process. This chapter discusses in detail the purification process of dextransferase. Dextransucrase produced by S. sanguis is a constituitive exocellular enzyme and can be purified starting with the culture fluids of late log or early stationary phase cells grown on glucose or other simple carbon sources. There is also a description of this enzyme's properties. The assay procedure of dextransucrase is described. Direct or indirect analyses may be employed for the measurement of dextransucrase activity for the assay procedure.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
