567 The protective effect of topical application of combination antioxidants in premature senescence of human skin

Abstract

The effect of ultraviolet (UV) radiation is well studied by increasing inflammatory response and oxidative stress leading to degradation of extracellular matrix (ECM) proteins such as collagen. Additionally, increasing numbers of studies have indicated the atmospheric pollution induces intracellular reactive oxygen species. Among various substances with potentials to prevent deleterious effect of environmental ageing, antioxidants are most representative and well studied, Especially, the combination formulation of L-ascorbic acid, vitamin E and Ferulic acid have shown it’s in vitro and in vivo effect in the prevention of photoaging. However, in presence, environmental ageing is induced by the additive effect of UV and air pollution. Therefore, in this study, we aimed to investigate the effect of UV and PM in human skin and examined whether the topical application of combination antioxidant formulation can potentially prevent and inhibit the signs of environmental skin ageing. We treated human dermal fibroblast in conditions to mimic environmental ageing; UVA, PM and UVA with PM in different dosages. The cell viability was determined using Cell Counting Kit-8. The expression levels of senescence-associated secretory phenotype (SASP) consisting of inflammatory cytokines and molecules were determined by qRT-PCR and western blot. To indicated the level of premature senescence, SA-β-gal staining was performed. Additionally, ex-vivo human skin samples were subject to UVA and PM to study the histologic change and immunohistochemical analysis in ECM marker expression. Our study suggests that topical application of combination antioxidants can be a promising candidate against environmental ageing.