Abstract
The Adeno-Associated Virus's (AAV) Inverted Terminal Repeats (ITRs) are key features of the AAV. ITRs are implied in the replication, encapsidation of the AAV genome, along with its integration in the host genome and its excision. These sequences are the only AAV DNA sequences conserved in the recombinant AAV as they allow its replication, encapsidation and long-term maintenance in the host non-dividing target cells. The ITRs are highly GC rich and structured due to the presence of repetitive palindromic sequences.During its replication, the AAV is able to reconstitute incomplete or imperfect ITR sequences. Thus it is often found that rAAV genomes cloned into the backbones of the DNA plasmids used for production, have imperfect ITRs sequences which are shortened or contain mutations.In this work, we study the impact of those imperfect ITRs and their impact on the levels of encapsidated rAAV genomes and cellular/baculovirus contaminating DNA sequences, in the baculovirus/Sf9 cells production system.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.