Abstract
Over the last decade, various protocols have been published to generate melanocytes from human pluripotent stem cells (CSP). However, there is limitations in their use specifically for developmental research studies and clinical applications in which a high purity of mel-CSP capable of high proliferative capacity is critical for a large-scale production of cells. To overcome the shortcomings associated to the previous protocols, we have successfully developed an efficient and robust stepwise method to generate a pure and homogenous melanocyte population with higher proliferate rate in culture.
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