555P - Association Between Colon Cancer Molecular Subtypes Obtained By Expression Profiling and By Microrna (Mir) Profiling

Abstract

ABSTRACT Aim: To analyze correlation between tumor subtypes obtained by expression profiling and by miR profiling in colon cancer and to identify miR targets in the colon tumors. Novel tumor subtypes have been identified in colon cancer with different clinical behavior. A gene signature associated with high-stroma recognized poor outcome patients (1). A similar classification associated high-stroma-subtype to an EMT-subtype and to a diferent response to cemotherapy treatment (2,3). Methods: mRNA and miR expression profiling were analyzed in 88 colorectal tumors (24 Dukes A, 26 B, 19 C, 19 D) using gene expression and miR microarrays. Hierarchical clustering was used to uncover tumor subtypes TALASSO software (http://talasso.cnb.csic.es) was used to find miR targets and their correlation wth mRNA expression. Pathways analysis was carried out using GeneCodis software (http://genecodis.cnb.csic.es). Results: There was a clear association of the tumor subtyes obtained by expression profiling wih the tumor subtypes obtained by miR profiling (p Conclusions: 1. There is a clear correlation of tumor classification obtained by expression profiling and by miR profiling 2. Stromal genes and BRAF mutations are asociated to both classifications 3. Pathways related to extracellular matrix and inflamation are implicated References 1-Perez-Villamil et al. BMC Cancer 2012 12:260 2- Schlicker A et al. BMC Medical Genomics 2012 5:66 3- Roepman P et al.Int J Cancer 2013 134:552. Disclosure: All authors have declared no conflicts of interest.