542 CHARACTERIZATION OF LUMBOSACRAL NEURAL ORGANIZATION OF MICTURITION REFLEX PATHWAYS WITH BLADDER DYSFUNCTION AFTER COMPLETE THORACIC SPINAL CORD TRANSECTION IN RATS

Abstract

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology (II)1 Apr 2013542 CHARACTERIZATION OF LUMBOSACRAL NEURAL ORGANIZATION OF MICTURITION REFLEX PATHWAYS WITH BLADDER DYSFUNCTION AFTER COMPLETE THORACIC SPINAL CORD TRANSECTION IN RATS Hai-Hong Jiang, Fu-Chen Yang, Brian Balog, Kevin Li, Ching-Yi Lin, Margot Damaser, and Yu-Shang Lee Hai-Hong JiangHai-Hong Jiang Cleveland, OH More articles by this author , Fu-Chen YangFu-Chen Yang Cleveland, OH More articles by this author , Brian BalogBrian Balog Cleveland, OH More articles by this author , Kevin LiKevin Li Cleveland, OH More articles by this author , Ching-Yi LinChing-Yi Lin Cleveland, OH More articles by this author , Margot DamaserMargot Damaser Cleveland, OH More articles by this author , and Yu-Shang LeeYu-Shang Lee Cleveland, OH More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1937AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bladder remodeling after spinal cord injury (SCI) causes related morphologic and functional changes, known as neurogenic bladder. Sacral nervous plasticity also occurs after SCI. These neuroplastic processes may relate to remodeling of the end organs of the bladder and the urethra; however the exact changes and patterns of neuroplasticity after SCI remain unknown. This study aims to identify micturition reflex network at lumbosacral and bladder dysfunction after T8 complete SCI using pseudoabies virus (PRV) transneuronal tracing technology. METHODS Eighty-five adult Sprague-Dawley female rats (225-275g) were used. All rats were divided into 3 groups: naive control, Tx-15 and Tx-45 (15 days and 45 days survival period after complete SCI procedure at the level of T8, respectively). Each group was further subdivided into 2 groups according to PRV injection to bladder body or external urethral sphincter (EUS). Three days after PRV injection to bladder or EUS, animals underwent perfusion followed by tissue harvest of bladder, urethra, and spinal cord. Thirty μm transverse sections were collected from the middle of L4 to the middle of S3 spinal segment for each sample. Animals in Tx-45 group also underwent urodynamic study 3 days before PRV injection. RESULTS Bladder remodeling occurs and becomes exacerbated 15 and 45 days after SCI according to the statistically significant changes of bladder weight. Bladder weight mirrors the bladder dysfunction. The main PRV-positive spinal segment from bladder injection of PRV after SCI moves distal with time from L6-S1 to S1-S2. The number of PRV-labeled neurons was positively correlated with bladder weight 15 days after bladder injection and 45 days after EUS injection, suggesting neuroplasticity after SCI occurs earlier in the pathway involving the bladder than in the pathway involving the urethra. CONCLUSIONS The window of time between bladder and urethra neuroplasticity after T8 complete SCI may indicate the time for full completion of micturition control pathway reorganization at the lumbosacral level. Further studies using specific synaptic stains in the spinal cord will be used to determine the synaptic plasticity and reorganization of the micturition reflex pathway after lumbosacral level SCI. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e222-e223 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hai-Hong Jiang Cleveland, OH More articles by this author Fu-Chen Yang Cleveland, OH More articles by this author Brian Balog Cleveland, OH More articles by this author Kevin Li Cleveland, OH More articles by this author Ching-Yi Lin Cleveland, OH More articles by this author Margot Damaser Cleveland, OH More articles by this author Yu-Shang Lee Cleveland, OH More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...