#5337 PHENOTYPIC CHANGES OF LYMPHOCYTES FOLLOWING RENAL TRANSPLANTATION

Abstract

Abstract Background and Aims Phenotypic changes in lymphocytes have been described following renal transplantation (RT), including both CD4 and CD8 subpopulations. The present work aims to prospectively assess of T lymphocyte phenotypic alterations, after RT and compare differences between deceased (DR) and living (LR) donor recipients. Method One hundred six RTRs were included in the study, 78/106 (74%) DR, age 52.5(15)yrs and 28/106 (26%) LD, 35(21)yrs. Lymphocytes, CD4, CD8, CD4CD28null, CD8CD28null, CD16+CD56+ (NK) and CD4+CD25+FoXP3+ (Tregs) were evaluated at peripheral blood, by flow cytometry, at certain time points: RT and 3, 6, 12 months (T0, T3, T6, T12, respectively). Results During follow up, at T3, T6, T12, eGFR was 61(23.6), 62.2(31.1), 63.5(23.9)ml/min/1.73 m2, p=NS and 59.1(24.8), 61(25.3), 62(22.7), p=NS, in DR and LR group, respectively. eGFR showed no significant difference between DR and LR at any time point. At any time point, T0, T3, T6, 12, lymphocytes were significantly increased in DR compared to LR groups, P = .001, p<0.0001, P = .002, p<0.0001, similarly CD4, P = .001, p<0.0001, P = .001, p<0.0001, and CD8 cells, P = .002, p<0.0001, P = .005, P = .006, respectively. Tregs were increased at T0, T3, T6, not at T12, p<0.0001, p<0.0001, P = .001, P = .1, respectively. At this point, Tregs were increased in RTRs with eGFR>50ml/min/1.73 m2, 20.3(9.3) vs. 27.2(18.6), P = .03. CD4CD28null, CD8CD28null cells did not change during follow up, and there was no difference between two groups at any time point. Conclusion Immune profile, was improved in both groups, DR or LR, however a significantly better effect managed in LR, although elimination of CD28 molecule could not be restored. Interestingly, Tregs were associated only by renal function one year following RT.