Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease with several key hallmarks, including elevated Th2 cytokines, interleukin (IL)-4 and IL-13; spongiosis; barrier deficits and pruritus. The goal of this study was to characterize the immune cell populations present in circulation in AD donors compared to healthy controls. Plasma samples from AD donors had increased levels of TARC (CCL17) and IgE consistent with active disease. Peripheral blood mononuclear cells (PBMC) isolated using standard Ficoll-Paque density gradient protocols were characterized using multi-color flow cytometry.

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