Abstract
Mesenchymal stem cells (MSCs) and embryonic stem (ES) cells are considered a source of cells for regenerative medicine and for cell and gene therapy. Efficient gene transfer into MSCs and ES cells is essential for basic research into cellular differentiation and developmental biology and for therapeutic applications in gene-modified regenerative medicine. In this study, we optimized the transduction of MSCs and ES cells by means of various types of adenovirus (Ad) vectors. We optimized fiber-modifications and promoter sequences. Following fiber-modified vectors were used: Ad vectors containing an RGD peptide in the HI loop of the fiber knob; Ad vectors containing a polylysine peptide in the C-terminal of the fiber knob; and Ad vectors containing a fiber protein derived from the Ad type 35. For optimization of the promoter sequences, EF-1|[alpha]|, CMV, CMV with intron A, and CA (|[beta]|-actin promoter/CMV enhancer) promoters were tested. An Escherichia coli |[beta]|-galactosidase (LacZ) gene was used as a reporter gene.
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