522 PROLACTIN-INDUCED PROTEIN (PIP) GENE IS POSSIBLY ASSOCIATED WITH THE PREPUCE DEVELOPMENT OF HYPOSPADIAS

Abstract

You have accessJournal of UrologyPediatrics: Congenital Anomalies - Lower Urinary Tract & Genitalia1 Apr 2010522 PROLACTIN-INDUCED PROTEIN (PIP) GENE IS POSSIBLY ASSOCIATED WITH THE PREPUCE DEVELOPMENT OF HYPOSPADIAS Satoshi Kurokawa, Yoshiyuki Kojima, Kentaro Mizuno, Hideyuki Kamisawa, Akihiro Nakane, Yutaro Hayashi, and Kenjiro Kohri Satoshi KurokawaSatoshi Kurokawa More articles by this author , Yoshiyuki KojimaYoshiyuki Kojima More articles by this author , Kentaro MizunoKentaro Mizuno More articles by this author , Hideyuki KamisawaHideyuki Kamisawa More articles by this author , Akihiro NakaneAkihiro Nakane More articles by this author , Yutaro HayashiYutaro Hayashi More articles by this author , and Kenjiro KohriKenjiro Kohri More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.721AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We have developed a rat model of hypospadias by exposure to flutamide in utero. This rat model showed proximal hypospadias in 100 % of offspring following maternal exposure to flutamide. We investigated the molecular mechanisms in the development of hypospadias at the critical period. We tried to identify genes that were down-regulated in hypospadiac tissue compared to control tissue. Genes may exist that have crucial roles in the development of hypospadias. We identified the Prolactin-induced protein (Pip) gene that may be especially associated with preputial development. METHODS Timed pregnant SD rats were administered 7.5 mg of flutamide or 1.5 ml of control vehicle by intraperitoneal injection from gestational days 15 to 17. Dams were sacrificed on gestational day 17, and the genital tubercles (GT) of male pups were harvested. Pip gene expression of extracted RNA was analyzed by quantitative RT-PCR and the distributions of Pip protein were examined histologically. Prepuces harvested from hypospadiac patients and buried penis patients (as a control) were used to analyze the Pip expression and distribution by quantitative RT-PCR and immunohistochemistry respectively. RESULTS The mRNA level of Pip was significantly lower in the hypospadiac GT than normal GT, and the Pip protein was locally expressed on the prepuce of GTs. The mRNA level of Pip was not significantly different in the prepuce of hypospadiac patients from that of control patients, but Pip was locally expressed on the prepuce of GTs. CONCLUSIONS To determine the hypospadiac development, it might be useful to investigate each aspect of three penile anormalies; an abnormal ventral opening of the urethral meatus, an abnormal ventral curvature of the penis and an abnormal distribution of prepuce. Pip, lower-expressed in the hypospadiac GT, may be associated with the prepuce development. The timing of harvesting prepuce from patients was different from the critical period of hypospadias development. This is one of the reasons why Pip gene expression was not different between the hypospadiac patients and the controls. Pip could be correlated with the abnormal development of hypospadiac prepuce. Nagoya, Japan© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e206 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Satoshi Kurokawa More articles by this author Yoshiyuki Kojima More articles by this author Kentaro Mizuno More articles by this author Hideyuki Kamisawa More articles by this author Akihiro Nakane More articles by this author Yutaro Hayashi More articles by this author Kenjiro Kohri More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...