Abstract
Our recent data described the placental microbiome as low biomass but ubiquitous in normal pregnancy, and further described taxa changes associated with spontaneous preterm birth (PTB). Histological descriptions of chorioamnionic bacteria in the absence of inflammation have been made by numerous other investigators, and thus far have been by gram staining and immunohistochemistry (techniques limited either in their exclusion of taxa or lack of specificity). Here we sought to quantitatively characterize the placental microbiome based on specific DNA probes using fluorescence in situ hybridization (FISH).
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