514 Effect of Topical Platelet Rich Plasma on Burn Healing After Partial Thickness Burn Injury

Abstract

Burn injury has direct impact on local and systemic changes. Many modalities for wound bed preparation was not satisfied. One of the most progressive dressing nowadays is PRP. PRP induces the synthesis of collagen with fibroblast. PRP accelerates wound healing and decrease the use of skin graft. Sixty Wistar albino rats were divided into 3 groups of 20 rats each. Group 1 (platelet-rich plasma group) was exposed to burn injury and topical platelet-rich plasma was applied. Group 2 (control group) was exposed to burn injury and normal saline was applied. Group 3 (blood donor group) was used as blood donors for platelet-rich plasma. Wound was excised on the fourteenth day then fixated in 10% formaldehyde. Specimen was then embedded in parafin block and stained with HE to observe the number of PMN and Masson’s Trichrome to observe collagen deposition. Collagen deposition was found significantly higher in Group 1 (platelet-rich plasma group) (P<0.05). The number of PMN is significantly less in Group 1 (P<0.05) meaning topical PRP also has anti-inflammatory effect. PRP is proven to accelerate burn healing after partial thickness burn injury by increasing the number of collagen deposition also having anti-inflammatory effect shown by the significantly less number of PMN in PRP group. PRP as a new method of burn wound dressing may be able to accelerate wound healing. In large burn area, PRP might help in decreasing the use of skin graft.