509 Genotoxic effects of cadmium chloride in V79 cell culture

Abstract

Cadmium chloride was tested for the ability to induce genotoxic effects in V79 cell culture. DNA damage induced by different doses of cadmium chloride was observed through the frequency of micronuclei in cytochalasin B-blocked micronucleus assay and through DNA migration in the single cell gel electrophoresis (comet assay). Cells were treated with different doses of cadmium chloride (10-4 to 10-6 M) 24 hours after seeding. The effects of ascorbic acid (conc. 100 microM) on cadmium-induced DNA damage was also evaluated. The results were analyzed by one-way ANOVA. The cytochalasin B-blocked micronucleus assay showed that 10-4 to 10-6 M and vitamin C supplemented 10-6 M frequencies of MN significantly differed from the control sample, and that 10-4, 10-5 M and vitamin C supplemented 10-5 M samples were significantly differed from the vitamin C sample. The Comet assay showed that the tail lengths from un-supplemented and vitamin C supplemented 10-4 M sample significantly differed from the control sample ; and that 10-5 M and vitamin C supplemented 10-4 M sample significantly differed from vitamin C sample. Tail moments from un-supplemented 10-4 M and vitamin C supplemented 10-6 M samples significantly differed from the vitamin C sample. Results indicate that cadmium chloride increases DNA damages as well as frequency of micronuclei. On the other hand, protective role of vitamin C was not confirmed at all tested concentrations of cadmium chloride.