507 CARBON NANOMATERIALS SENSITIZE PROSTATE CANCER CELLS TO MITOMYCIN C AND DOCETAXEL

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research (III)1 Apr 2013507 CARBON NANOMATERIALS SENSITIZE PROSTATE CANCER CELLS TO MITOMYCIN C AND DOCETAXEL Jessica Ringel, Kati Erdmann, Kai Kraemer, Susanne Fuessel, and Manfred P. Wirth Jessica RingelJessica Ringel Dresden, Germany More articles by this author , Kati ErdmannKati Erdmann Dresden, Germany More articles by this author , Kai KraemerKai Kraemer Dresden, Germany More articles by this author , Susanne FuesselSusanne Fuessel Dresden, Germany More articles by this author , and Manfred P. WirthManfred P. Wirth Dresden, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1901AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Carbon nanomaterials such as carbon nanofibers (CNFs) and carbon nanotubes (CNTs) represent an innovative strategy to increase the anticancer activity of conventional chemotherapeutics. In this study, we investigated the ability of CNTs and CNFs to sensitize prostate cancer (PCa) cells to the chemotherapeutics mitomycin C (MMC) and docetaxel (DTX). METHODS DU145 PCa cells were treated with CNFs, CNTs, MMC or DTX alone as well as in combination for 24 h. For the combinations, the cells were first treated with carbon nanomaterials alone for 22 h, afterwards the respective chemotherapeutic was added for another 2 h. Effects of the different treatments on cell viability were assessed using WST-1 assay. Inhibition of cell growth was determined by measuring cell counts and rate of apoptosis. The replicative potential of the treated cell population was determined by the clonogenic assay. Cellular uptake of CNFs and CNTs was investigated by microscopy studies. RESULTS CNFs and CNTs showed low to moderate cytotoxicity and were internalized by PCa cells. The addition of either CNFs or CNTs to the chemotherapeutics clearly enhanced their cytotoxic effects. MMC alone reduced the viability of DU145 cells to 68%, whereas a combined treatment with MMC and CNFs led to a decrease to 20%. DTX alone resulted in a decrease of viability to 94%, combined treatment with CNF led to less than 30% remaining viability. This synergistic enhancement of the combined treatment with either chemotherapeutic and CNFs depended on the concentration of CNFs. Cell death caused by apoptosis was induced more prominently in combinations than in single treatments, e.g. a combination of MMC and CNFs more than doubled the apoptosis rate. The cell count as well as the ability to form cell colonies was more reduced in the combinatory treatments with CNFs. Combinations of either chemotherapeutic with CNTs showed similar cellular effects but were less pronounced than those with CNFs. CONCLUSIONS The innovative carbon nanomaterials CNFs and CNTs were able to enhance the cytotoxic effects of the chemotherapeutics MMC and DTX on PCa cells without possessing intrinsic toxicity. Interactions between the cellular membrane and carbon nanomaterials could possibly lead to an enhanced accumulation of the chemotherapeutics. Carbon nanomaterials-based applications could present a new strategy to overcome chemoresistance by sensitizing cancer cells to conventional chemotherapeutics. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e208 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jessica Ringel Dresden, Germany More articles by this author Kati Erdmann Dresden, Germany More articles by this author Kai Kraemer Dresden, Germany More articles by this author Susanne Fuessel Dresden, Germany More articles by this author Manfred P. Wirth Dresden, Germany More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...