505 DEVELOPMENT OF AN ANTICANCER HYPERTHERMIC THERAPY FOR BONE-SPECIFIC METASTASIS IN PROSTATE CANCER BY USING ANTI-RANKL ANTIBODY-CONJUGATED MAGNETIC NANOPARTICLES

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research (III)1 Apr 2013505 DEVELOPMENT OF AN ANTICANCER HYPERTHERMIC THERAPY FOR BONE-SPECIFIC METASTASIS IN PROSTATE CANCER BY USING ANTI-RANKL ANTIBODY-CONJUGATED MAGNETIC NANOPARTICLES Noriyasu Kawai, Kaname Tsutsumiuchi, Takashi Kobayashi, Daichi Kobayashi, Toshiki Etani, Taku Naiki, Ryosuke Ando, Keiichi Tozawa, and Kenjiro Kohri Noriyasu KawaiNoriyasu Kawai Nagoya, Japan More articles by this author , Kaname TsutsumiuchiKaname Tsutsumiuchi Kasugai, Japan More articles by this author , Takashi KobayashiTakashi Kobayashi Kasugai, Japan More articles by this author , Daichi KobayashiDaichi Kobayashi Nagoya, Japan More articles by this author , Toshiki EtaniToshiki Etani Nagoya, Japan More articles by this author , Taku NaikiTaku Naiki Nagoya, Japan More articles by this author , Ryosuke AndoRyosuke Ando Nagoya, Japan More articles by this author , Keiichi TozawaKeiichi Tozawa Nagoya, Japan More articles by this author , and Kenjiro KohriKenjiro Kohri Nagoya, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1899AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We had recently developed magnetite nanoparticles (MNPs) for inducing intracellular hyperthermia. MNPs generate heat under an alternating magnetic field (AMF). In MNP-mediated hyperthermia therapy, the MNPs are irradiated using AMF at a frequency at which the MNPs generate heat; this approach ensures that heating occurs only in the carcinoma tissues. We refer to this technique as ′MNP+AMF heat therapy.′ In this study, we developed a super selective hyperthermic therapy for bone metastasis of prostate cancer using ′MNP+AMF heat therapy.′ with anti-RANKL antibody-conjugated MNPs. METHODS Anti-RANKL antibody was conjugated with MNPs by using N-cyclohexyl-N '-(2-morpholinoethyl) carbodiimide. Cellular uptakes were determined in mouse osteoblasts (MC3T3-E1), which contain RANKL, by using anti-RANKL antibody-conjugated MNPs and, anti-HER-2 antibody-conjugated MNPs as controls. We investigated the effect of antibody-conjugated MNP+AMF hyperthermia in vitro in nude mice bone metastatic model of prostate cancer. which was established by injection of rat prostate cancer cell:PCai1 around the spine bone. We injected anti-RANKL antibody-conjugated MNPs via tail vein and irradiated AMF for hyperthermia,which MNPs reached supine bone metastatic lesion by anatomical relation. RESULTS In MC3T3-E1 cells, the uptake of anti-HER 2 and anti-RANKL antibody-conjugated MNPs was 1.2 and 3.5 pg/cell, respectively, which indicated anti-RANKL antibody-conjugated MNPs much adhered to MC3T3-E1 cells than those of anti-HER 2. We confirmed the adhesion of anti-RANKL antibody-conjugated MNPs to supine bone metastatic lesion by histochemical analysis of sacrificed specimens. Temparature of bone metastatic lesion elevated by irradiation. This result clearly indicated the effect of the hyperthermic potentiation induced by the anti-RANKL antibody-conjugated MNPs. CONCLUSIONS The newly synthesized antibody-conjugated MNP can be considered as a novel magnetite nanoparticle for targeting cancer cells. The anti-RANKL antibody-conjugated MNPs might be effective for specific hyperthermia therapy of the bone-specific metastasis in prostate cancer. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e207 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Noriyasu Kawai Nagoya, Japan More articles by this author Kaname Tsutsumiuchi Kasugai, Japan More articles by this author Takashi Kobayashi Kasugai, Japan More articles by this author Daichi Kobayashi Nagoya, Japan More articles by this author Toshiki Etani Nagoya, Japan More articles by this author Taku Naiki Nagoya, Japan More articles by this author Ryosuke Ando Nagoya, Japan More articles by this author Keiichi Tozawa Nagoya, Japan More articles by this author Kenjiro Kohri Nagoya, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...