Abstract
"2-Color Laser-induced Fluorescence" (2-C LIF) is a novel quantitative temperature measurement method in microfluidic flow. A conventional method (1-Color LIF) measurement causes spatial random error which depends on spatio-temporal distribution of excitation light intensity. In contrast, the proposed method can cancel the undesirable influence using a ratio-metric technique with two fluorescence images, because fluorescence intensity can be approximately a linear function of the excitation light intensity. Therefore, it is possible to measure the temperature distribution quantitatively and simplify temperature calibration. In this paper, we represent the application of this method for micro-electrophoretic flow. We applied to calibrate between the temperature and ratio value which is calculated by dividing the Rhodamine B fluorescence image with the Rhodamine 110 fluorescence image. As a result, spatial intensity error can be reduced to about 90% as compared with 1-Color LIF image. We visualized temperature distribution in micro-electrophoreticwith high resolution 0.24μm×0.24μm and sensitivity 1.32%/K.
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