Abstract
Publisher Summary This chapter discusses the antiproliferative assay for human interferons. One of the tumorigenic clones, C#9 is used. The growth inhibitory activity of the interferons is measured using a 7-9 day assay comparing the growth of the cells that received (a) nothing additional, (b) the diluents of the interferons, and (c) the different interferon samples at various concentrations. It is of importance that several concentrations of the interferon be used over an extended period in the assay. As can be seen, the greatest changes, particularly between different concentrations, occur at a period of 5 to 9 days after the interferon is introduced. This method has allowed us to distinguish between the antigrowth and cytotoxic effects of the interferons. This assay has been found to be useful in measuring the antigrowth potential of purified interferon, as well as recombined interferons for their relative antiviral and antigrowth activities; these studies show the antiviral and antigrowth activities are not identical. The antiviral activity is measured using the assay from Roche based on reduction of cytopathic effect. The various human cell lines in the assay system are tested and have found that the T-24-9C line preferable to the others due to its ease of manipulation and high responsiveness.
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