Abstract
Double-stranded DNA antibody detection is important in the diagnosis and management of patients with systemic lupus erythematosus. Current radioimmunoassay and haemagglutination methods for determining DNA antibodies are technically complicated or produce varying results between laboratories. A simple inexpensive indirect immunofluorescent method using fixed human metaphase chromosomes for DNA antibody estimation is described. The method has been compared to the currently available Crithidia luciliae method and the Farr radioimmunoassay technique. The specificity of the metaphase method has been confirmed by showing that only DNAase enzyme treatment abolishes reactivity. RNAase and trypsin have no effect. The human metaphase method showed 100% correlation with a positive Farr assay in 38 out of 373 routine DNA antibody estimations. Simultaneous testing using the Crithidia method showed 84% correlation. Testing 46 ANA positive sera for DNA antibody using Crithidia and metaphase methods showed highly significant correlation of results ( p
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
