Abstract
CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydrogenase) and flavoprotein D-fructose dehydrogenase in the membranes. When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield was realized with the resting cells. The method proposed here should give a smart strategy for 5-keto-D-fructose production.
Highlights
When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield was realized with the resting cells
D-fructose, with G. oxydans strain having chromo some-integrated fdh of G. japonicas NBRC 3260 and invertase gene of G. japonicus LMG 1417 [10]
In general cases of the oxidative fermentation catalyzed by the cytoplasmic system of acetic acid bacteria, D-sorbitol is oxidized predominantly to L-sorbose and D-mannitol to D-fructose
Summary
Gluconobacter frateurii CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydro genase) and flavoprotein D-fructose dehydrogenase in the membranes. KEYWORDS Acetic acid bacteria; Gluconobacter frateurii; 5-keto-D-fructose; oxidative fermentation Regarding to 5-keto-D-fructose (5KF) production by acetic acid bacteria, the first report was done in 1960 by Terada et al.
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