Abstract
CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydrogenase) and flavoprotein D-fructose dehydrogenase in the membranes. When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield was realized with the resting cells. The method proposed here should give a smart strategy for 5-keto-D-fructose production.
Highlights
When the two enzymes are functional, D-mannitol is converted to 5-keto-D-fructose with 65% yield when cultivated on D-mannitol. 5-Keto-D-fructose production with almost 100% yield was realized with the resting cells
D-fructose, with G. oxydans strain having chromo some-integrated fdh of G. japonicas NBRC 3260 and invertase gene of G. japonicus LMG 1417 [10]
In general cases of the oxidative fermentation catalyzed by the cytoplasmic system of acetic acid bacteria, D-sorbitol is oxidized predominantly to L-sorbose and D-mannitol to D-fructose
Summary
Gluconobacter frateurii CHM 43 have D-mannitol dehydrogenase (quinoprotein glycerol dehydro genase) and flavoprotein D-fructose dehydrogenase in the membranes. KEYWORDS Acetic acid bacteria; Gluconobacter frateurii; 5-keto-D-fructose; oxidative fermentation Regarding to 5-keto-D-fructose (5KF) production by acetic acid bacteria, the first report was done in 1960 by Terada et al.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
