Abstract
BackgroundPrevious Positron Emission Tomography (PET) studies of 5-HT1A receptors have shown an influence of several genetic factors, including the triallelic serotonin transporter gene-linked polymorphic region on the binding potential (BPND) of these receptors. The aim of our study was to investigate the relationship between a 5-HT1A promoter polymorphism and the binding potential of another selective 5-HT1A receptor antagonist, [18F]MPPF, in healthy subjects.MethodsThirty-five volunteers, including 23 women, underwent an [18F]MPPF scan and were genotyped for both the C(-1019)G 5-HT1A promoter polymorphism and the triallelic serotonin transporter gene-linked polymorphic region. We used a simplified reference tissue model to generate parametric images of BPND. Whole brain Statistical Parametric Mapping and raphe nuclei region of interest analyses were performed to look for an association of [18F]MPPF BPND with the C(-1019)G 5-HT1A promoter polymorphism.ResultsAmong the 35 subjects, 5-HT1A promoter genotypes occurred with the following frequencies: three G/G, twenty-one G/C, and eleven C/C. No difference of [18F]MPPF BPND between groups was observed, except for two women who were homozygote carriers for the G allele and showed greater binding potential compared to other age-matched women over the frontal and temporal neocortex. However, the biological relevance of this result remains uncertain due to the very small number of subjects with a G/G genotype. These findings were not modified by excluding individuals carrying the S/S genotype of the serotonin transporter gene-linked polymorphic region.ConclusionsWe failed to observe an association between the C(-1019)G 5-HT1A promoter polymorphism and [18F]MPPF binding in healthy subjects. However our data suggest that the small number of women homozygote for the G allele might have greater [18F]MPPF BPND relative to other individuals. This finding should be confirmed in a larger sample.
Highlights
Previous Positron Emission Tomography (PET) studies of 5-HT1A receptors have shown an influence of several genetic factors, including the triallelic serotonin transporter gene-linked polymorphic region on the binding potential (BPND) of these receptors
The binding potential (BP) of [11C]WAY100635 at 5HT1A receptors is reported to be influenced by several genetic factors, including the serotonin transporter genelinked polymorphic region (5-HTTLPR) [1,2]
We have recently evaluated the impact of this polymorphism on the BP of another selective 5-HT1A receptor antagonist
Summary
Previous Positron Emission Tomography (PET) studies of 5-HT1A receptors have shown an influence of several genetic factors, including the triallelic serotonin transporter gene-linked polymorphic region on the binding potential (BPND) of these receptors. The binding potential (BP) of [11C]WAY100635 at 5HT1A receptors is reported to be influenced by several genetic factors, including the serotonin transporter genelinked polymorphic region (5-HTTLPR) [1,2]. We have extended our research to examine a possible association between C(-1019)G 5HT1A promoter polymorphism and [18F]MPPF BP This polymorphism is located in the regulatory region of the 5-HT1A promoter which inhibits transcriptional repression of the 5-HT1A gene [9,10], and is part of a 26 base pair imperfect palindrome [11]. This palindromic region is recognized by two transcription factors; the nuclear deformed epidermal autoregulatory factor (NUDR, Deaf1) and Hes. The G allele is supposedly associated with higher expression of 5-HT1A receptors in the raphe nuclei and decreased 5-HT release, consistent with the association reported between G/G genotype and major depression [11,12]
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