5-HT 7 receptor deletion enhances REM sleep suppression induced by selective serotonin reuptake inhibitors, but not by direct stimulation of 5-HT 1A receptor

Abstract

5-HT 7 receptors are involved in REM sleep and possibly in mood disorders. REM sleep suppression and antidepressant-like behavior is observed in 5-HT 7 −/− mice and in rats treated with 5-HT 7 receptor antagonists. We recently demonstrated that pharmacological blockade of 5-HT 7 receptors enhances REM sleep suppression and antidepressant-like behavior induced by citalopram in rodents. It has been hypothesized that the effect of citalopram on sleep is essentially mediated by the activation of 5-HT 1A receptors. The present study investigates the impact of 5-HT 7 receptor gene deletion on the effect of various reuptake inhibitors on REM sleep and probes the role of 5-HT 1A receptors in this response. Three SSRIs (citalopram, fluoxetine and paroxetine) but not the tricyclic antidepressant desipramine had a significantly stronger REM sleep suppressive effect in 5-HT 7 −/− mice compared to 5-HT 7 +/+ mice. In contrast, REM sleep was similarly reduced in 5-HT 7 +/+ mice and 5-HT 7 −/− mice after treatment with the 5-HT 1A receptor agonist ipsapirone. Furthermore, both 5-HT 7 +/+ and 5-HT 7 −/− mice displayed the same increase in REM sleep duration produced by the 5-HT 1A receptor antagonist WAY-100635. These findings indicate that 5-HT 7 receptor deletion augments the effect of various SSRIs on REM sleep suppression and that this effect is distinct from those mediated via 5-HT 1A receptors.