Abstract
目的：探讨去甲基化药物5-杂氮-2＇-脱氧胞苷（5-Aza-CdR）在裸鼠体内诱导HepG2细胞T-cadherin的表达,及其对HepG2种植瘤生长的抑制作用. 方法：皮下接种法建立HepG2细胞裸鼠种植瘤模型,随机分为实验组（11只）和对照组（10只）.实验组裸鼠给予5-Aza-CdR注射,对照组裸鼠注射等量PBS.4wk后处死裸鼠,观察种植瘤的生长情况,用RT-PCR技术及免疫组织化学技术检测T-cadherin mRNA及蛋白的表达. 结果：用药4wk后,实验组肿瘤组织的T-cadherin mRNA的表达水平显著高于对照组（t=6.613,P〈0.05）;对照组HepG2细胞几乎检测不到T-cadherin蛋白表达,而实验组裸鼠种植瘤细胞膜上可检测到T-cadherin蛋白表达;实验组肿瘤的平均体积明显小于对照组肿瘤平均体积（t=2.337,P=0.025）. 结论：5-Aza-CdR能诱导裸鼠皮下种植HepG2瘤细胞的T-cadherin表达,抑制HepG2种植瘤的生长,其机制可能为5-Aza-CdR使甲基化的T-cadherin启动子去甲基化,恢复T-cadherin的表达,从而抑制HepG2种植瘤生长.
Published Version
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