Abstract
Aim To determine whether 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is effective in combating ultraviolet A- (UVA-) induced oxidative photodamage of hairless mice skin in vivo and human epidermal keratinocytes in vitro. Methods In in vitro experiments, the human keratinocyte cell line (HaCaT cells) was divided into two groups: the experimental group was treated with ALA-PDT and the control group was left untreated. Then, the experimental group and the control group of cells were exposed to 10 J/m2 of UVA radiation. ROS, O2− species, and MMP were determined by fluorescence microscopy; p53, OGG1, and XPC were determined by Western blot analysis; apoptosis was determined by flow cytometry; and 8-oxo-dG was determined by immunofluorescence. Moreover, HaCaT cells were also treated with ALA-PDT. Then, SOD1 and SOD2 were examined by Western blot analysis. In in vivo experiments, the dorsal skin of hairless mice was treated with ALA-PDT or saline-PDT, and then, they were exposed to 20 J/m2 UVA light. The compound 8-oxo-dG was detected by immunofluorescence. Conclusion In human epidermal keratinocytes and hairless mice skin, UVA-induced oxidative damage can be prevented effectively with ALA-PDT pretreatment.
Highlights
E Skin photodamage has become a major public health issue given the tremendous increase in the number of patientsRsuffering from it
reactive oxygen species (ROS), O2− species, and Membrane Potential (MMP) were determined by fluorescence microscopy; p53, R OGG1, and xeroderma pigmentosum group C (XPC) were determined by Western blot analysis; apoptosis was determined by flow cytometry; and 8-oxo-dG was determined by immunofluorescence
The levels of SOD2 protein medical cotton was soaked with a specific concentration of in the aminolevulinic acid-based photodynamic therapy (ALA-PDT) group increased by 64.94%, 86.36%, and
Summary
A- (UVA-) induced oxidative photodamage of hairless mice skin in vivo and human epidermal keratinocytes in vitro. The human keratinocyte cell line (HaCaT cells) was divided into two groups: the experimental group was treated with ALA-PDT and the control group was left untreated. R OGG1, and XPC were determined by Western blot analysis; apoptosis was determined by flow cytometry; and 8-oxo-dG was determined by immunofluorescence. SOD1 and SOD2 were examined by Western blot analysis. The dorsal skin of hairless mice was treated with ALA-PDT or saline-PDT, and they were exposed to 20 J/m2 UVA light. In human epidermal keratinocytes and hairless mice skin, UVA-induced oxidative damage can be prevented.
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