496 The Role of Selenium as an Antioxidant in Protecting Against Free Radical Damage in Renal Cell Carcinoma

Abstract

Abstract Aim To explore the effects of Selenium as an antioxidant on renal cell carcinoma cell lines (RCC). Method RCC cell lines under strict culture conditions were divided into two groups: control (group A) and experimental groups. Experimental groups were Selenium treated (group B), exposed to oxidative stress induced with H2O2 (group C), and oxidative stress induced with H2O2 followed by Selenium treatment (group D) and selenium treatment followed by oxidative stress induced by H2O2 (group E). Changes in the cell growth and its survival was evaluated in each group after 72 hours. Evaluation of the ROS was done using Cellular Reactive Oxygen Species Detection Assay Kit (Abcam; ab186029), and cell viability was measured using MTT reagent (Sigma; 11465007001). Results The generation of the ROS was significantly high in groups C (125.4% ± 14.6), D (132.9% ± 16.6) and E (135.3% ± 22.1) when compared to group A (100% ± 14) and B (91.7% ± 14.6). Moreover, the RCC cell viability was (98.7% ± 10.8) in group B compared to C, D and E (80.8% ± 5.4, 79.7%± 5.9 and 74.8% ± 8.8; respectively). Moreover, an association was observed between viability of cells and ROS generation. The higher the ROS generation (group C, D and E), the lower was its viability. Likewise, the lower ROS generation (group B), the more viable were the cells. Conclusions The decreased generation of ROS in selenium treated group coupled with increased viability of cancer cells indicates the negative prognostic effect of selenium on RCC cells.