496 Differential chromatin accessibility and gene expression suggest Th17 polarization in activated and skin-homing T cells

Abstract

We generated 1,090 ATAC-seq and 1,057 T-cell RNA-seq libraries from 153 subjects, derived from 8 flow-sorted T-cell subsets (defined by CD4/CD8, CLA+/ CLA-, and 0/24h CD3/CD28 stimulation). Effects of activation and skin-homing were analyzed by DESeq2. After peak calling, 78,234 consensus peaks were present in ≥ 30 ATAC-seq libraries. A Wald test examining simple main effects identified 9,072, 3,934, and 21,174 consensus peaks as differentially accessible regions (DAR; FDR < 0.05, |log2 FC|≥ 0.585) in CD4 vs CD8, CLA+ vs CLA-, and resting vs.