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Abstract
The time of initial synthesis and the localization of the glial fibrillary acidic (GFA) protein have been studied in the spinal cord white matter of the chick embryo from the 9th day of incubation until hatching with the indirect immunofluorescence method. The appearance of GFA protein on the 12th day of incubation in the marginal zones of the anterior columns was preceded by a marked increase of neuroglial density in the same areas. The next day, immunofluorescence gradually extended laterally and posteriorly in the marginal zones of the lateral columns adjacent to the leptomeninges. In the posterior columns, immunofluorescence was first observed on the 16th day; a peripheral distribution was not apparent. On the 21st day, the day of hatching, immunofluorescence was uniformly distributed in all three columns but not so intense as in a 13-day-old chicken.
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