49 INFLUENCE OF DIFFERENT LEVELS OF NEONATAL ASPHYXIA IN BASIC NEONATAL CHEMISTRY IN RATS

Abstract

AIM:We studied the effects of different levels of hypoxia (see table) on basic biochemical markers from newborn rat brain to assess the relation between asphyxia intensity and biochemical outcome.MATERIAL AND METHODS: Three groups of newborn Wistar rats were submitted 15 minutes to two different levels of hypoxia (air and N2 gas mixture) and the third one was exposed just to an air flow, then they were sacrificed. The brain was removed and frozen in liquid N2 in less than 1 minute. After being weighted brains were homogenized and enzymatic tests for Lactate (LAC), Pyruvate (PIR), Glucose (GLU) and Lactic dehydrogenase were performed. Kruskall-wallis test was applied as non-parametric distribution was found. (Mean ± SD).CONCLUSIONS:Results showed that groups O2=2l and O2 =5 had closer biochemical profiles than group O2 =l0 did with each other. We can conclude that non proportional relation exists between the degree of, hypoxia with the basic biochemical outcome during short hypoxic exposition periods.