Abstract
Publisher Summary Oxalic acid may be extracted from tissues by standard methods of maceration or homogenization with dilute hydrochloric acid solution. In virtually all methods of oxalate determination, precipitation with calcium ions is the method of choice. The highly insoluble calcium oxalate may be determined directly by weighing, either as such, or after conversion to calcium oxide, sulfate, or carbonate by ignition. Most convenient, however, is solution in dilute mineral acid and titration with potassium permanganate. The method of determination of glycolic acid involve analyses of acid in the conical centrifuge tube in the presence of 0.01% solution of 2,7- dihydroxynaphthalene in concentrated sulfuric acid. The color obtained is read with a photoelectric colorimeter at 530 mμ and compared with a standard curve prepared in the usual manner. The method may also be used for quantitative estimation of oxalic and glyoxylic acids by reducing these to glycolic acid with magnesium powder.
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