#4845 EFFECT OF OMEGA-3 FATTY ACID ON MITOCHONDRIAL MEMBRANE FATTY ACID AND COMPARISON WITH ERYTHROCYTE MEMBRANE IN ADENINE INDUCED UREMIC RATS

Abstract

Abstract Background and Aims The kidney has the second highest mitochondrial content in the human body. Fatty acids (FAs) are one of the important energy sources and main constituents of cell membranes. Higher erythrocyte membrane oleic acid contents are related to acute coronary syndrome and omega-3 FA can reduce oleic acid contents. We investigated whether omega-3 FA modifies not only erythrocyte membrane FA but also mitochondrial membrane FA of kidney in adenine-induced uremic rats. Method Male Sprague-Dawley rats were fed diets containing 0.75% adenine and 2.5% protein for three weeks. Next, rats were randomly divided into six groups that were fed diets containing 2.5% protein and saline with cholecalciferol (3000 IU/kg/week) or omega-3 FAs (300 mg/kg/day) with cholecalciferol were supplemented by gastric gavage for four weeks: normal control (n = 7), adenine control scarified at 3 weeks (n = 6), adenine control scarified at 5 weeks (n = 5), adenine control scarified at 7 weeks (n = 5), omega-3 FAs group scarified at 5 weeks (n = 5), and omega-3 FAs group scarified at 7 weeks (n = 5). The mitochondrial isolation kit was used for renal mitochondria extraction. The mitochondrial and erythrocyte membrane FA contents were measured using gas chromatography. Results Compared to the normal control group, serum creatinine levels in adenine control group was significantly increased and improved in omega-3 FA group. Compared with adenine control, erythrocyte and mitochondrial membrane monounsaturated FA contents including oleic acid and arachidonic acid (AA) levels were significantly decreased in omega-3 FA group. FA compositions were similar between erythrocytes and mitochondrial membranes in each group. Monounsaturated FA contents including oleic acid, and eicosapentaenoic acid were higher and saturated FA was lower in the mitochondrial membrane than erythrocyte membrane in adenine control group. Conclusion Omega-3 FA affects not only erythrocyte membrane FA but also mitochondrial membrane FA in uremic rats. Erythrocyte membrane FA contents can reflect mitochondrial membrane FA contents of the kidney.