482. Neuronal Gene Transfer with HIV-1-Based Lentiviral Vectors Pseudotyped with Lyssavirus Glycoproteins

Abstract

Background: The delivery of therapeutic proteins to the central nervous system represents an appealing strategy for the treatment of nerve injury and disorders of the CNS. Important factors determining CNS targeting include tropism of the viral vectors and retrograde transport of the vector particles. In the present study, an HIV-1-based lentiviral vector was pseudotyped with four different envelope (Env) glycoproteins generating four different HIV-1 pseudotypes. To select a high tropism HIV-1 pseudotype for CNS targeting, SHSY-5Y neuroblastoma cells were infected with pseudotyped vectors.