48] Kinetic studies of immobilized α-chymotrypsin in aprotic solvents

Abstract

Publisher Summary This chapter discusses immobilized chymotrypsin and other immobilized proteolytic enzymes, such as immobilized trypsin and immobilized plasmin. The uses of immobilized enzymes are manifold. Some are commercial and others are academic. The chapter describes the two of academic uses. The first is the use of immobilized α-chymotrypsin in kinetic studies of the enzyme in dipolar aprotic organic solvents that have led to a more precise description of the catalytic mechanism of α-chymotrypsin action. The other use that has found for immobilized α-chymotrypsin is in denaturation. The chapter develops a method in which a proteolytic enzyme can be immobilized on a glass surface without any diminution of its catalytic activity. This was accomplished by covalently attaching the enzyme at a sufficient distance from the surface so that there is no interaction whatsoever between the enzyme and the surface to which it is attached.