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Abstract
OUR PREVIOUS METHODS for assaying equine gonadotropin (1, 2) involved autopsy of the test rats 120 hours after injection. The primary purpose for this long test interval was to obtain information on the qualitative ovarian response in follicular growth, luteinization, and interstitial cell development. This information was considered essential because, conceivably, the gonadotropic activity of pregnant mare serum might depend upon two or more gonadotropic hormones. We therefore wished to compare the nature of the responses obtained from sera collected at different intervals during pregnancy as well as the responses of various fractions obtained during studies on methods of purification. After comparing the qualitative and quantitative gonad response to extracts testing 4,000 to 7,000 R. U. per mg. total solids, with the response to untreated mare serum in normal and hypophysectomized male and female rats, we concluded that mare serum contains but one gonadotropic hormone (3). Three papers (4–7), give evid...
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