Abstract
We previously described the cloning and expression of canine methylguanine-DNA methyl transferase (MGMT) using monocistronic HIV-1 and gamma-retroviral vectors (Zaboikin M et. al. (2004) Hum Gene Ther 15:383-392). In that study, we showed that the canine MGMT was as efficient as the human MGMT for providing resistance to nitrosourea drugs such as BCNU. Here, we describe the creation and testing of dual-gene expression HIV-1 vectors encoding canine MGMT and EGFP under control of the EF1α promoter.
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