457 REGULATORY T CELLS IN CLINICALLY LOCALIZED RENAL CELL CARCINOMA: COMPARISON WITH AN AGE-MATCHED HEALTHY CONTROL

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You have accessJournal of UrologyKidney Cancer: Basic Research (III)1 Apr 2013457 REGULATORY T CELLS IN CLINICALLY LOCALIZED RENAL CELL CARCINOMA: COMPARISON WITH AN AGE-MATCHED HEALTHY CONTROL Taekmin Kwon, In Gab Jeong, Yunlim Kim, Jong Hyun Yoon, Kwang Hyun Kim, Dalsan You, Jun Hyuk Hong, Hanjong Ahn, and Choung-Soo Kim Taekmin KwonTaekmin Kwon Seoul, Korea, Republic of More articles by this author , In Gab JeongIn Gab Jeong Seoul, Korea, Republic of More articles by this author , Yunlim KimYunlim Kim Seoul, Korea, Republic of More articles by this author , Jong Hyun YoonJong Hyun Yoon Seoul, Korea, Republic of More articles by this author , Kwang Hyun KimKwang Hyun Kim Seoul, Korea, Republic of More articles by this author , Dalsan YouDalsan You Seoul, Korea, Republic of More articles by this author , Jun Hyuk HongJun Hyuk Hong Seoul, Korea, Republic of More articles by this author , Hanjong AhnHanjong Ahn Seoul, Korea, Republic of More articles by this author , and Choung-Soo KimChoung-Soo Kim Seoul, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1848AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES There has been increasing evidence for focusing on the role of regulatory T (Treg) cells in cancer immunity. However, the literature regarding the frequency of regulatory T cells (Treg) in renal cell carcinoma (RCC) compared with normal control is controversial. Also, there is increasing evidence for age-dependent development of Treg cells. We investigated the frequency of Tregs in the peripheral blood of RCC patients compared with age-matched healthy control and compared the prevalence of Treg cells in the tumor infiltrating lymphocytes (TIL) with lymphocyte in normal kidney tissue. METHODS The frequency of Treg cells in the peripheral blood of 62 patients with clinically localized RCC and from 65 healthy controls was evaluated by flow cytometry using specific monoclonal antibodies recognizing CD4(+), CD25 (+) and Foxp3 (+) markers. The prevalence of Treg cells in the TIL and lymphocyte in normal kidney tissue from 54 patients with RCC were also evaluated. RESULTS The median age of the RCC and control group was 55 (<40 years: n=8, 40-49: n=13, 50-59: n=16, ≥60: n=25) and 53 (<40 years: n=9, 40-49: n=18, 50-59: n=16, ≥60: n=22) years, respectively. The median tumor size was 6.0cm (1.7-13.7cm) and 49 patients (79%) had clear cell histology on pathologic examination. There was no difference in the frequency of Treg cells in the peripheral blood in entire RCC patients compared with healthy donors (2.8 ± 4.0% vs 1.9 ± 2.6%, p=0.150). Treg cells frequency was found to significantly higher in RCC patients with large tumor size (≥7cm, 4.2 ± 5.5%) than those from normal healthy control or small to medium sized RCC patients (<7cm, 2.0 ± 2.6%, p=0.016). Furhman nuclear grade did not affect the frequency of Treg cells among RCC patients (Gr 1-2: 2.5 ± 3.6% vs Gr 3-4: 3.0 ± 4.4%, p=0.675). There was no statistically significant difference in the prevalence of Treg cells between TIL and lymphocyte in normal kidney tissue (5.5 ± 9.5% vs 4.8 ± 5.8%, p=0.629). CONCLUSIONS We provide evidence of an increased pool of Treg cells in the peripheral blood of clinically localized RCC patients with substantial tumor burden compared with age-matched healthy control. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e187-e188 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Taekmin Kwon Seoul, Korea, Republic of More articles by this author In Gab Jeong Seoul, Korea, Republic of More articles by this author Yunlim Kim Seoul, Korea, Republic of More articles by this author Jong Hyun Yoon Seoul, Korea, Republic of More articles by this author Kwang Hyun Kim Seoul, Korea, Republic of More articles by this author Dalsan You Seoul, Korea, Republic of More articles by this author Jun Hyuk Hong Seoul, Korea, Republic of More articles by this author Hanjong Ahn Seoul, Korea, Republic of More articles by this author Choung-Soo Kim Seoul, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...