Abstract
Alkyldihydroxyacetonephosphate (alkyl-DHAP) synthase catalyzes the biosynthesis of an ether bond in different animal systems. This membrane bound enzyme is mainly present in peroxisomes of animals and was originally described to be in guinea pig liver mitochondrial fractions and rat brain microsomes. This chapter describes the method by which the guinea pig liver enzyme is solubilized and partially purified. The method is based on the separation of the radioactive ionic product, 1- O -[1 ' - 14 C]hexadecyl-DHAP from the nonionic radioactive precursor, [1- 14 C]hexadecanol, by solvent partition in a chloroform–methanol–water system at high pH. Thereagents used are glycolic acid (dreid by keeping overnight over P 2 0 5 under reduced pressure in a desiccator), pyridine (dried over CaH 2 ), chloroform (dried by by distilling from P 2 O 5 ), palmitoyl chloride, diazald, 85% phosphoric acid, and anhydrous diethyl ether..
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
