444 In vitro and clinical improvement of sensitive skin functions by ADE-G3 formulations

Abstract

Sensitive skin is a common condition that can severely impact quality of life. Several mechanisms are thought to be involved, including those affecting the skin barrier function, hydration and skin innervation. Our Goal was to investigate clinically and on in vitro models, the benefit of formulations dedicated to sensitive skin and containing Aquaphilus dolomiae extract-G3 (ADE-G3) on skin barrier function and hydration, as well as protective responses to dry and pollution stresses. In vitro sensitized reconstructed human epidermis (RHE) were subjected to dehydration and pollution stress with or without the formulations. Endpoint measurements included transepithelial electric resistance (TEER), and protein expression. Clinical measurements included transepithelial water loss (TEWL), skin pH, the lipid index, and hydration scores. When applied topically in formulations, ADE-G3, increased the TEER in sensitized RHEs. In dehydrated RHEs, formulations increased recovery of skin barrier integrity, evident as a return of the ratios of filaggrin/pro-filaggrin and Caspase-14/pro-Caspase-14 to values measured in control RHEs, as well as the “natural moisturizing factors”. In clinical studies, performed on dry human skin, the formulations helped to improve the skin barrier function and the level of hydration as maintained over 24 h after application. This was evident as an intense and sustained moisturization, with total lipids and lipid esters increase, an increase in pH, and a decrease in the TEWL. Moreover, when exposed to pollution stress by treating the RHE with benzo[a]pyrene and airborne particulate matter (PM10), application of formulations prior to exposure attenuated the induction of CYP1A1, CYP1B1 and UGT1A7 expression, indicating a protective effect. ADE-G3 formulations increased the hydration of the skin but also protected and improved the skin barrier integrity of sensitive skin exposed to dry and cold and airborne pollutant induced stress environments.