Abstract
Vesicular stomatitis virus (VSV) is extensively employed for assaying interferon preparations. There are several advantages in employing VSV in interferon assays including that VSV is very sensitive to the effect of interferon, and therefore, even small quantities of interferon in a given sample can be measured, the virus can replicate and form plaques in a variety of cells from vertebrate and invertebrate origin, the virus grows quickly and plaques can be enumerated within 24-hour after the infection; thus the time required is considerably shorter than with other viruses and thus a comparison of interferon preparations from different sources can be made and VSV grows to high titer in several cell types. VSV is a negative-strand virus belonging to the rhabdovirus group. The infectivity of a VSV preparation is most commonly assayed by plaque formation. The principle of this method is that the spread of the virus released from the infected cells is restricted to neighboring cells by including agar in the medium.
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