44. CAN PGT-A USING ARRAY CGH IMPROVE IVF CLINICAL RESULTS? (THE EGYPTIAN IVF-ET CENTER EXPERIENCE)

Abstract

Introduction Chromosomal aneuploidies are considered the main cause of failed development of embryos, implantation failures, and early reproductive losses. Preimplantation genetic testing (PGT) using array comparative genomic hybridization (array-CGH) allows selection of embryos with normal karyotype, before embryo transfer. This should increase the implantation rate and reduce the frequency of early pregnancy loss. The aim of this study is to assess if PGT using array-CGH improve IVF clinical results. Material & methods During 2016 - 2018, a total of 97 cycles for 94 patients underwent blastomere biopsy by laser on day 3, and 90 fresh transfer cycles were performed. The indications were repeated IVF failure (30), repeated pregnancy loss (26), previous history of aneuploid offspring (10) and for aneuploidy screening and sex selection (28). The maternal ages ranged from 25 to 42yrs. 732 embryos from 97 cycles were subjected to day-3 biopsies, the DNA was amplified using modified whole genome amplification (WGA) protocol. The amplified DNA from 710 embryos was labeled and analyzed using array-CGH software (IBSA Genetics., Switzerland). Results 710 embryos was tested, 69.44% (493/710) of the embryos were aneuploid267, and 30.46% (217/710) were euploid.70 euploid embryos were cryopreserved, and 147 euploid embryos were transferred to 90 patients resulting in 50% (45/90) pregnancies, 22 deliveries with healthy babies and 20 ongoing pregnancies and 3 miscarriages. Conclusion: Our results show that array CGH has the potential to provide high pregnancy and delivery rates after transfer of euploid embryos.