439 Probiotic Effect of the Regulation of Innate Immune Response, dc and Adaptive Cellular Immune Response and the Balance TH1, TH2, TREG Through Sensors TLR-2 AND TLR-4, on the Intestinal Mucosa in BALB/C Health Status and Balb/C Status of Exposure to LPS

Abstract

BackgroundThe concept of improving host defense as a preventive effort in the face of exposure to pathogens through the gut mucosal immune system must be developed where the normal intestinal flora plays an important role. Probiotics through various research can improve intestinal mucosal immune system, but so far the effect of probiotics on the regulation of innate immune responses of dendritic cells and adaptive cellular immune response as well as the balance of TH1, TH2, TREG through TLR-2 sensors and TLR-4 on intestinal mucosa Balb/c healthy status and Balb/c LPS exposure status is unclear. The purpose is to examine the effect of probiotics against dendritic cells regulation of innate immune responses and adaptive cellular immune response as well as the balance TH1, TH2, TREG through TLR-2 sensors and TLR-4, on the intestinal mucosa in Balb/c health status and Balb/c status of exposure to LPS.MethodsMale Balb/c divided into 4 treatment groups. Two groups given probiotics for 21 days, one group will be given exposure to LPS on day 15. One group will exposure by LPS alone and one group as control group without treatment. All groups terminated after a day-to-21. Immunohistochemical examination of ileal mucosa using monoclonal antibodies specific for dendritic cells, TLR-2, TLR-4, IL-1, IL-2, IL-4, IL-6, IL-10, IL-2, TNFα and TGFß.ResultsIn LPS group there were downregulation both innate and cellular immune system indicates the occurrence of adaptive homeostasis disorders. In the group receiving probiotics there were upregulation both innate and cellular immune system is adaptive to indicate an alert. When the probiotic group exposured by LPS, it was still maintained by the improvement of the balance indicate TREG immune system remains in a state of homeostasis.ConclusionsProbiotics can improve alertness status innate and cellular adaptive immune mucosa in healthy mice and can maintain the balance of TH1, TH2 and TREG so that homeostasis is maintained.