436 IDENTIFICATION OF UNIQUE GENOMIC PROFILES OF COLLECTING DUCT CARCINOMA

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research III1 Apr 2012436 IDENTIFICATION OF UNIQUE GENOMIC PROFILES OF COLLECTING DUCT CARCINOMA Toru Inoue, Keiko Matsuura, Chisato Nakada, Takeo Nomura, Fuminori Sato, Yoji Nagashima, Chikara Ohyama, Tomonori Habuchi, Yasuhiko Tomita, Satoru Takahashi, Osamu Ogawa, Norio Nonomura, Akio Matsubara, Masayuki Nakagawa, Masatsugu Moriyama, and Hiromitsu Mimata Toru InoueToru Inoue Yufu, Japan More articles by this author , Keiko MatsuuraKeiko Matsuura Yufu, Japan More articles by this author , Chisato NakadaChisato Nakada Yufu, Japan More articles by this author , Takeo NomuraTakeo Nomura Yufu, Japan More articles by this author , Fuminori SatoFuminori Sato Yufu, Japan More articles by this author , Yoji NagashimaYoji Nagashima Yokohama, Japan More articles by this author , Chikara OhyamaChikara Ohyama Hirosaki, Japan More articles by this author , Tomonori HabuchiTomonori Habuchi Akita, Japan More articles by this author , Yasuhiko TomitaYasuhiko Tomita Yamagata, Japan More articles by this author , Satoru TakahashiSatoru Takahashi Tokyo, Japan More articles by this author , Osamu OgawaOsamu Ogawa Kyoto, Japan More articles by this author , Norio NonomuraNorio Nonomura Osaka, Japan More articles by this author , Akio MatsubaraAkio Matsubara Hiroshima, Japan More articles by this author , Masayuki NakagawaMasayuki Nakagawa Kagoshima, Japan More articles by this author , Masatsugu MoriyamaMasatsugu Moriyama Yufu, Japan More articles by this author , and Hiromitsu MimataHiromitsu Mimata Yufu, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.503AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Collecting duct carcinoma (CDC) is a relatively rare subtype of renal cell tumors. It is sometimes difficult for pathologists to distinguish CDC from high grade papillary renal cell carcinoma (PRCC), mucinous tubular and spindle cell carcinoma (MTSCC) and invasive urothelial carcinoma (UC) of renal pelvis. Aim of this study is to analyze the genomic profiles by array comparative genomic hybridization (aCGH) and clarify whether aCGH is useful for the differential diagnosis of CDC. METHODS 9 cases of CDC, 4 cases of MTSCC, 3 cases of high grade PRCC and 14 cases of invasive UC were analyzed. Carcinoma cells were collected from paraffin-embedded tissue sections by laser-captured microdissection and subjected to aCGH using Whole Human Genome 4x44K Oligo Micro Array (Agilent). RESULTS Array CGH analysis revealed that, in more than 50% of cases of CDC, gene copy number gains at 17q and 8q and losses at 1p, 3p, 4pq, 6q, 9pq, 11q, 13q, 14q, 18q and 22q were detected (Figure). The pattern of genomic profiles of CDC was evidently distinct from those of UC and papillary RCC. Unsupervised hierarchical cluster analysis of the aCGH data of 30 cases revealed that they were divided into 3 groups (Clusters A, B and C). Cluster A contained 13 cases including 8 cases of CDC, 3 cases of MTSCC and 2 cases of UC, indicating that most of CDCs and MTSCCs were categorized in the cluster A. Cluster B contained 3 cases of papillary RCC, one case of MTSCC, one case of UC and one case of CDC. On the other hand, Cluster C contained only 11 cases of UC. Interestingly, 8 of 9 cases of CDC was categorized in Cluster A and 3 of the 4 cases of MTSCC were also categorized in Cluster A, suggesting that genomic profiles of CDC may be distinct from those of UC and papillary RCC, but genomic profiles of MTSCC cannot be discriminated from those of CDC. CONCLUSIONS The patterns of CNAs of CDC are unique and different from those of the other histologic subtypes except for MTSCC. On the basis of the pattern of CNAs, MTSCC is suggested to be classified as a CDC-related subtype. Genomic profiling using aCGH is useful for the differential diagnosis of CDC, high grade papillary RCC and invasive UC. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e179 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Toru Inoue Yufu, Japan More articles by this author Keiko Matsuura Yufu, Japan More articles by this author Chisato Nakada Yufu, Japan More articles by this author Takeo Nomura Yufu, Japan More articles by this author Fuminori Sato Yufu, Japan More articles by this author Yoji Nagashima Yokohama, Japan More articles by this author Chikara Ohyama Hirosaki, Japan More articles by this author Tomonori Habuchi Akita, Japan More articles by this author Yasuhiko Tomita Yamagata, Japan More articles by this author Satoru Takahashi Tokyo, Japan More articles by this author Osamu Ogawa Kyoto, Japan More articles by this author Norio Nonomura Osaka, Japan More articles by this author Akio Matsubara Hiroshima, Japan More articles by this author Masayuki Nakagawa Kagoshima, Japan More articles by this author Masatsugu Moriyama Yufu, Japan More articles by this author Hiromitsu Mimata Yufu, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...