Abstract
Background: A novel antimicrobial peptide derived from insulin-like growth factor-binding protein 5 (AMP-IBP5) displays both antimicrobial and immunomodulatory properties. However, the role of AMP-IBP5 in the regulation of skin barrier remains unclear. Objective: To investigate the effects of AMP-IBP5 on skin barrier function. Methods: Normal human epidermal keratinocytes were stimulated with AMP-IBP5 and Western blot was used to analyze the expression of tight junction (TJ)-related proteins. Immunofluorescence staining was used to examine the intercellular distribution of TJ-related proteins.
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