Abstract
Publisher Summary This chapter describes the preparation of squalene epoxidase from rat liver microsomes. The squalene epoxidase system is comprised of the terminal oxidase, which is distinct from hemoproteins such as cytochrome P-450 isozymes, 2 and a flavoprotein identical with NADPHcytochrome P-450 reductase. Principle and procedure of the assay method are described. In principle of assay method, the activity of squalene epoxidase can be determined in the reconstituted system of NADPH-cytochrome P-450 reductase and squalene epoxidase by measuring the formation of radioactive 2,3-oxidosqualene from [14C]squalene. The specific activity of the labeled squalene can be adjusted by mixing with unlabeled squalene, which was purified through the thiourea clathrate. 6 The assay incubation is carried out at 37 ° in a Pyrex culture tube (12 ml).
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