Abstract
Publisher Summary This chapter describes the quantitative determination of noncovalently bound flavins. Three types of procedures are in general use for the analysis of mixtures containing FAD, FMN, and riboflavin. These include: (1) spectrophotometric analysis, (2) enzymic analysis, and (3) fluorometric analysis. Distinction between the covalently bound and noncovalently bound flavins is made by the fact that the former require treatment of proteins with which they are associated with agents that can hydrolyze peptide bonds and the latter are released into solution by agents that affect the tertiary and quarternary protein structures. Riboflavin 5'-phosphate (flavin mononucleotide, FMN) and the phosphoanhydride between FMN and adenosine 5'-phosphates (flavin adenine dinucleotide, FAD) are the flavin compounds most commonly found in biological materials. Other flavins, including riboflavin itself, hydroxylated derivatives of FMN and FAD, flavin glycosides, and a number of covalently bound FAD derivatives are also found in small quantities in tissue extracts or in particular purified enzymes.
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