Abstract
Keratinocyte (KC) culture medias are used interchangeably with the expectation of comparable results. The COVID-19 pandemic resulted in supply chain shortages necessitating substitutions to standard protocols. We screened available medias on the KC cell line N/TERT2G and found biological responses varied considerably across three culture medias: KSFM (Gibco, #17005042), KGM2 (PromoCell, #C-20211), and Defined (Gibco, #10744019). We observed qualitative and quantitative differences in proliferation among KC grown in the different medias; KC cultured in Defined had a significantly lower proliferative capacity.
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