406: Amniotic fluid derived cells: genome-wide expression in early and late cultures

Abstract

expression in early and late cultures Ashwin Jadhav, Jiri Zavadil, Ross Basch, Bruce Young UMDNJ-RWJMS, Obstetrics and Gynecology, New Brunswick, NJ, New York University Medical Center, Obstetrics and Gynecology, New York, NY, New York University Medical Center, Department of Pathology, New York, NY OBJECTIVE: Evaluates change in the genome wide expression profile of Amniotic Fluid Stem Cells during in-vitro culture STUDY DESIGN: Amniotic Fluid Stem Cells (AFSC) expressing CD117 (c-kit) surface markers were sorted using magnetic beads technique, mRNA extracted and converted to cDNA and in vitro transcribed to biotin-labeled cDNA using the standard Affymetrix 3’IVT Express Kit protocol. The labeled cDNA hybridization to the Affymetrix U133 2.0 arrays, array fluidics processing and scanning were performed under standardized conditions recommended by Affymetrix. Analysis of the microarray expression profile was performed using genespring multiomics software. RESULTS: We found that only 3.08% of gene probes were differentially expressed from early to late passage of AFSC culture. The differentially expressed genes were related to biological processes or cellular function including transcription factors, protein kinases, and cytokines/ growth factors. Other gene-sets of interest were oncogenes and tumor suppressor genes, which were a very small number of genes. We further analyzed the gene sets of interest using NIH DAVID and GSEA bioinformatics databases for gene annotations analysis. Applying false discovery rate correction, there was no significant difference in the genome-wide expression profiling between early and late passage. CONCLUSION: Amniotic fluid derived stem cells (AFSCs) maintain their genome-wide expression profile during in-vitro culture.