404.5: The Role of LAG3 in Antibody Responses to Kidney Transplantation

Abstract

The functions of coinhibitory receptor lymphocyte activation gene-3 (LAG3) in T cells are well studied, however its role in humoral immune responses remains poorly characterized. The goal of this study was to test the role of recipient LAG3 in a mouse model of renal allograft rejection. Murine kidney transplant surgeries were performed from C3H (H-2Dk) donors to B6 WT, LAG3-/-, CD4CreLAG3fl/fl & CD19CreLAG3fl/fl recipients (H-2Db) after bilateral nephrectomy. Grafts were evaluated by immunistochemistry. WT recipients were treated with anti-mCD20 or anti-CD8 mAbs to deplete B cells or CD8 T cells, respectively. Immune responses were assessed by flow cytometry, ELISPOT assay, and serum levels of MHC-I and MHC-II-reactive IgG donor specific antibody (DSA) were determined by ELISA. Compared to WT animals, naïve B6.LAG3-/- mice have elevated numbers of CD44hi memory T cells, CXCR5hi follicular T cells, and B220+CD138+ plasma cells, and increased frequencies of memory T cells reactive to H-2Dd, H-2Ds, H-2Dq and H-2Dk (Figure 1A) and increased levels of serum IgG antibodies against H-2Dd, H-2Dk, I-Ad and I-Ak alloantigens (Figure 1B). All C3H kidney allografts survived for > 60d (n=5) in WT recipients, whereas recipient LAG3 deficiency led to rapid allograft rejection (MST of 14d, n=5) (Figure 2A) and elevated serum creatinine levels at d14 posttransplant. Compared to WT, LAG3-/- recipients had elevated frequencies of anti-donor IFNγ producing T cells and increased levels of DSA against MHC-I and MHC-II (Figure 2B). Graft histology at rejection revealed minimal T cell infiltration, diffuse C4d staining, atrophic peritubular capillaries, endothelial swelling and edema characteristic of antibody mediated rejection (AMR) (Figure 2C). Recipient CD8 T cell depletion did not alter rejection kinetics in LAG3-/- recipients (MST of 16d) (Figure 3A&B), and graft histological findings (Figure 3C) mirrored those of unmanipulated LAG3 deficient recipients (Figure 2C), demonstrating hallmark characterstics of AMR. B cell depletion significantly extended C3H kidney allograft survival (MST of >30d) (Figure 3D&E), suggesting the predominant role of alloantibody rather than T cell mediated rejection in these mice. However, neither T nor B cell conditional knockout recipients rejected the allograft demonstrating LAG3 expression on both cell types is necessary to mediate rejection.These findings demonstrate that LAG3 regulates both T and B cell functions in response to kidney allografts, and is an attractive therapeutic target for the prevention of AMR.