Abstract
Development of a safe and effective nonviral gene vector remains a significant challenge in the field of gene therapy. Rational design of more efficient gene carriers will be possible only with mechanistic insights of the rate-limiting steps in the nonviral gene transfer process. In order to examine the intracellular transport of DNA nanoparticles and their dissociated individual components, we have labeled nanoparticles with quantum dots (QD) and fluorescent organic dyes as a donor and acceptor pair for fluorescence resonance energy transfer (FRET).
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